Journal: Cell Death & Disease

Article Title: Role and dynamics of vacuolar pH during cell-in-cell mediated death

doi: 10.1038/s41419-021-03396-2

Figure Lengend Snippet: The pH of vacuoles containing live or dead inner cells ( A ), and inner cell fates ( B ), and the ways inner cells died (entotic or apoptotic) ( C ), for MCF10A cells upon treatments with EN6 (10 μM), CQ (20 μM), ConA (200 nM), and NH 4 Cl (100 μM). n (left to right) = 30, 33, 30, 31, 30 for A ; 96, 98, 89, 94, 104 for B ; 150, 129, 76, 65, 60 for C . ** p < 0.01; *** p < 0.001. D Representative images of CICs, with inner cells undergoing entotic death (up) or apoptotic death (low), that were stained with antibody for cleaved-caspase 3 (cyan). Keima for merged channels of 440 and 550 nm. White arrows indicate inner cells. Scale bar, 10 μm. E Quantification of inner cells that were positive in cleaved-caspase 3, or died either entotically or apoptotically. n (left to right) = 64, 57, respectively. The pH of vacuoles containing live or dead inner cells ( F – H ), and inner cell fates ( I – K ), for cells (MCF7, MM231/E, and SW480/E) upon treatments with EN6 (10 μM), CQ (20 μM), ConA (200 nM), and NH 4 Cl (100 μM), respectively. n (left to right) = 26, 23, 26, 20, 28 for F ; 31, 32, 57, 38, 33 for G ; 36, 43, 32, 37, 32 for H ; 89, 78, 96, 80, 85 for I ; 117, 118, 159, 160, 108 for J ; 120, 108, 104, 134, 141 for K . * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Reagents including EN6 (Selleck, #S6650), Hydroxychloroquine (CQ) (MCE, #HY-W031727), Concanamycin A (ConA) (Shanghai ZZbio.co, ZAE-ALX-380-034-C025), NH4Cl (Coolaber, CA30112320), LysoTracker (Invitrogen, #L7526), DAPI (Life technologies, #D1306) were purchased and used according to manufacturer’s instructions.

Techniques: Staining

Journal: Journal of Experimental Botany

Article Title: Pollination induces autophagy in petunia petals via ethylene

doi: 10.1093/jxb/ers395

Figure Lengend Snippet: Microscopy analysis of senescing petals of petunia flowers after pollination. (A) Flowers pollinated at anthesis are shown at 0, 1, 2, and 3 d after pollination (dap). Bars, 1cm. (B) Electron micrographs of mesophyll cells in the petals of petunia flowers. Detached flowers were pollinated or not at anthesis and treated with concanamycin A. Mesophyll cells located in the middle between vascular bundles in the petals of unpollinated flowers (left) and pollinated flowers (right) at 2 d after anthesis are shown. The spherical body indicated by an arrow is shown in an inset at higher magnification. Bars, 5 µm (main pictures); 500nm (inset). (C) MDC staining in mesophyll cells of petunia petals. Petal limbs collected from flowers at 0, 1, 2, and 3 dap were stained. A micrograph of the petal limb at 2 dap taken at higher magnification is shown as an inset. Bars, 500 µm (main pictures); 50 µm (inset).

Article Snippet: For concanamycin A (Wako, Osaka, Japan) treatment, detached flowers were placed in 5 µM concanamycin A solution in vials and then pollinated.

Techniques: Microscopy, Staining

Journal: Journal of Experimental Botany

Article Title: Pollination induces autophagy in petunia petals via ethylene

doi: 10.1093/jxb/ers395

Figure Lengend Snippet: Effects of concanamycin A treatment on dry weight and nitrogen content in pollinated flowers. Detached flowers were pollinated at anthesis and placed in distilled water (Cont) or 5 µM concanamycin A (ConcA) for 3 d. Dry weights of petals, ovaries, and receptacle with sepals (Rec+sep) were measured for each flower ( n =30). Nitrogen (N) content was determined for three sets of ten tissues ( n =3). Each bar represents the mean±SE. Asterisks indicate a significant difference at P <0.05 (*) by Student’s t -test.

Article Snippet: For concanamycin A (Wako, Osaka, Japan) treatment, detached flowers were placed in 5 µM concanamycin A solution in vials and then pollinated.

Techniques: